Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8751722 | Virus Research | 2018 | 5 Pages |
Abstract
Porcine galectin-3 (GAL3) is a 29-kDa protein encoded by a single gene, LGALS3, located on chromosome 1. Here, using a yeast two-hybrid screen of a cDNA library from porcine alveolar macrophage cells (PAMs), we report for the first time that GAL3 interacts with nonstructural protein 12 (Nsp12) of the porcine reproductive and respiratory syndrome virus (PRRSV). Although extensive research has focused on porcine reproductive and respiratory syndrome (PRRS), little is known about the pathogen and host interactions involving individual nonstructural viral proteins, especially Nsp12. Here, we showed that GAL3 interacted with viral Nsp12 following co-transfection of HEK293 cells with GAL3- and Nsp12-expressing plasmids. Additionally, we observed that PPRSV infection led to reduced GAL3 levels during the late phase of infection in both MARC-145 cells and PAMs. Importantly, GAL3 overexpression significantly suppressed the replication of both type 1 and 2 PRRSV strains, whereas knockout of endogenous LGALS3 in MARC-145 cells significantly increased viral titer and expression of the nucleocapsid protein. These results strongly support a direct inhibitory effect of GAL3 on PRRSV replication, which might contribute to an overall antiviral effect. Furthermore, our findings provide insights into the molecular basis of the role Nsp12 plays in PRRSV pathogenesis.
Keywords
hpiPorcine alveolar macrophagePAMPRRSVGAL3NSPhpt2′,5′-oligoadenylate synthetaseMOISTATinterferonIFNhours post-infectionCo-IPnonstructural proteinSignal transducer and activator of transcriptionknockoutCo-ImmunoprecipitationOASWestern blotPorcine reproductive and respiratory syndrome virusN proteinNucleocapsid proteinmultiplicity of infectionGalectin-3
Related Topics
Life Sciences
Immunology and Microbiology
Virology
Authors
Liwei Li, Yanjun Zhou, Yifeng Jiang, Fei Gao, Tongling Shan, Kuan Zhao, Yujiao Zhang, Lin Li, Guangzhi Tong,