Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8751748 | Virus Research | 2018 | 6 Pages |
Abstract
White spot syndrome virus immediate early (IE) gene wsv249 encodes an E3 ubiquitin ligase that can interact with a shrimp ubiquitin-conjugating enzyme to mediate ubiquitination. In this study, to understand the transcriptional regulation of wsv249, a serial of 5â²-truncated mutations were made on its promoter and the activities of mutated promoters was analyzed. Four 25â¯bp regions potentially containing either positive or negative regulatory elements were identified. Notably, the deletion of â275/â250, which abolished a cAMP-response element (CRE), greatly reduced the promoter activity by 84.2%. CRE serves as the binding site for proteins belong to the cAMP responsive element-binding proteins (CREBs) family and the activator protein 1 (AP-1) family. Electrophoretic mobility shift assay (EMSA) showed that Lvc-Jun could directly bind to the CRE element in the promoter region of wsv249. In addition, the regulation of shrimp homolog of c-Jun and CREB on wsv249 promoter was further investigated. We found that Lvc-Jun greatly upregulated the activity of wsv249 promoter by â¼12.4 fold, and the CRE at â212/â205 but not the one at â256/â249 was essential for the regulation. In contrast, LvCREB-3 could not activate wsv249 promoter activity. These findings extend our knowledge of the transcriptional regulation of WSSV IE genes.
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Authors
Zi Yang, Xiaomin Xu, Fang Li, Feng Yang,