Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8751800 | Virus Research | 2018 | 8 Pages |
Abstract
Tomato black ring virus (TBRV) is the only member of the Nepovirus genus that is known to form defective RNA particles (D RNAs) during replication. Here, de novo generation of D RNAs was observed during prolonged passages of TBRV isolates originated from Solanum lycopersicum and Lactuca sativa in Chenopodium quinoa plants. D RNAs of about 500â¯nt derived by a single deletion in the RNA1 molecule and contained a portion of the 5â² untranslated region and viral replicase, and almost the entire 3â² non-coding region. Short regions of sequence complementarity were found at the 5â² and 3â² junction borders, which can facilitate formation of the D RNAs. Moreover, in this study we analyzed the effects of D RNAs on TBRV replication and symptoms development of infected plants. C. quinoa, S. lycopersicum, Nicotiana tabacum, and L. sativa were infected with the original TBRV isolates (TBRV-D RNA) and those containing additional D RNA particles (TBRVâ¯+â¯D RNA). The viral accumulation in particular hosts was measured up to 28 days post inoculation by RT-qPCR. Statistical analyses revealed that D RNAs interfere with TBRV replication and thus should be referred to as defective interfering particles. The magnitude of the interference effect depends on the interplay between TBRV isolate and host species.
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Virology
Authors
Beata Hasiów-Jaroszewska, Julia Minicka, Aleksandra ZarzyÅska-Nowak, Daria BudzyÅska, Santiago F. Elena,