Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8751844 | Virus Research | 2018 | 30 Pages |
Abstract
African swine fever virus (ASFV) causes a contagious and frequently lethal disease of pigs causing significant economic consequences to the swine industry. The ASFV genome encodes for more than 150 genes, but only a few of them have been studied in detail. Here we report the characterization of open reading frame L83L which encodes a highly conserved protein across all ASFV isolates. A recombinant ASFV harboring a HA tagged L83L protein was developed (ASFV-G-L83L-HA) and used to demonstrate that L83L is a transiently expressed early virus protein. A recombinant ASFV lacking the L83L gene (ASFV-G-ÎL83L) was developed from the highly virulent field isolate Georgia2007 (ASFV-G) and was used to show that L83L is a non-essential gene. ASFV-G-ÎL83L had similar replication in primary swine macrophage cells when compared to its parental virus ASFV-G. Analysis of host-protein interactions for L83L identified IL-1β as its host ligand. Experimental infection of domestic pigs showed that ASFV-G-ÎL83L is as virulent as the parental virus ASFV-G.
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Authors
Manuel V. Borca, Vivian O'Donnell, Lauren G. Holinka, Elizabeth RamÃrez-Medina, Benjamin A. Clark, Elizabeth A. Vuono, Keith Berggren, Marialexia Alfano, Lucas B. Carey, Juergen A. Richt, Guillermo R. Risatti, Douglas P. Gladue,