Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8783735 | Reproductive BioMedicine Online | 2018 | 39 Pages |
Abstract
Upon freeze-thawing or vitrification-warming, and assessment of donor-derived spermatogenesis after transplantation, Dulbecco's modified Eagle's medium supplemented with 1.5M dimethyl-sulphoxide, 10% fetal calf serum and 60âµM of Z-VAD-(OMe)-FMK in vials at a freezing rate of â1°C/min was optimal.
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Authors
Jaime Onofre, Katrien Faes, Prashant Kadam, Elena Vicini, Ans M.M. van Pelt, Ellen Goossens,