| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8817600 | Biomedical and Environmental Sciences | 2017 | 6 Pages |
Abstract
We studied effects of nutrient quercetin on cytochromes' P450 1A (CYP1A) activities (measured spectrofluorimetrically using 7-ethoxy-resorufin for CYP1A1 and 7-methoxy-resorufin for CYP1A2 as substrates), on mRNA levels (measured by RT-PCR), and on DNA-binding activities (evaluated by an electrophoretic mobility shift assay) of proteins regulating CYP1A expression in untreated and benzo(α)pyrene (BaP)-treated rats. Wistar rats received quercetin, BaP, or both once daily for 1-3 days. Quercetin did not influence CYP1A1 in untreated rats but inhibited BaP-mediated CYP1A induction on the transcriptional level decreasing positive input (AhR functional activity) and increasing negative input (AhRR/ARNT expression and Oct-1 and C/EBP functional activities).
Related Topics
Health Sciences
Medicine and Dentistry
Public Health and Health Policy
Authors
ML Perepechaeva, TA Seredina, YA Sidorova, EN Pivovarova, AL Markel, VV Lyakhovich, AY Grishanova,