Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8817607 | Biomedical and Environmental Sciences | 2017 | 5 Pages |
Abstract
In this study, we isolated a virus strain (YN12031) from specimens of Armigeres subalbatus collected in the China-Laos border. BHK-21 cells infected with YN12031 exhibited an evident cytopathic effect (CPE) 32 h post-infection. The virus particles were spherical, 70 nm in diameter, and enveloped; they also featured surface fibers. Molecular genetic analysis revealed that YN12031 was closely related to alpha viruses such as Chikungunya virus and Sindbis virus, and located in the same clade as MM2021, the prototype of Getahvirus (GETV) isolated in Malaysia in 1955. Phylogenetic analysis of the E2 and capsid genes further revealed that YN12031 was located in the same clade as the Russian isolate LEIV/16275/Mag. Analysis of the homology of nucleotides and amino acids in the coding area and E2 gene demonstrated that the YN12031 isolated from the China-Laos border (tropical region) was related closest to the LEIV/16275/Mag isolate obtained in Russia (North frigid zone area) among other isolates studied. These results suggest that GETV can adapt to different geographical environments to propagate and evolve. Thus, strengthening the detection and monitoring of GETV and its related diseases is very crucial.
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Authors
Yuan Yuan LI, Shi Hong FU, Xiao Fang GUO, Wen Wen LEI, Xiao Long LI, Jing Dong SONG, Lei CAO, Xiao Yan GAO, Zhi LYU, Ying HE, Huan Yu WANG, Xiao Jie REN, Hong Ning ZHOU, Gui Qin WANG, Guo Dong LIANG,