Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8827348 | Transplantation Proceedings | 2018 | 6 Pages |
Abstract
The assay had a minimum detection limit of 10 copies/μL and a strong linear standard curve (y = â3.3682x + 38.594; R2 = 0.999) within the range of the input CAR gene (10-107 copies/μL). The reproducibility test showed a coefficient of variation ranging from 0.63%-1.65%. Real-time qPCR is a highly sensitive, specific, reproducible, and universal method that can be used to detect anti-CD19 CAR-T cells in peripheral blood.
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Authors
H. Wang, X. Du, W.-H. Chen, J. Lou, H.-L. Xiao, Y.-M. Pan, H. Chen, N. An, Q.-X. Zhang,