Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8840188 | Journal of Neuroscience Methods | 2018 | 13 Pages |
Abstract
We suggest that the standard extended culture periods for primary neuronal cultures, coupled with the requirement for successive medium changes, may leave them particularly susceptible to cumulative albumin contamination from the culture media used. Finally, we propose the implementation of simple alterations to wash steps in protein extraction protocols which can ameliorate this interference.
Related Topics
Life Sciences
Neuroscience
Neuroscience (General)
Authors
Ashleigh Willis, Judith A. Pratt, Brian J. Morris,