Article ID Journal Published Year Pages File Type
8840188 Journal of Neuroscience Methods 2018 13 Pages PDF
Abstract
We suggest that the standard extended culture periods for primary neuronal cultures, coupled with the requirement for successive medium changes, may leave them particularly susceptible to cumulative albumin contamination from the culture media used. Finally, we propose the implementation of simple alterations to wash steps in protein extraction protocols which can ameliorate this interference.
Related Topics
Life Sciences Neuroscience Neuroscience (General)
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