Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8842037 | Neuroscience Research | 2018 | 9 Pages |
Abstract
Secretagogin (SCGN) is a recently discovered calcium binding protein of the EF hand family, cloned from β cells of pancreatic island of Langerhans and endocrine cells of the gastrointestinal gland. SCGN characterizes some particular neuron groups in various regions of the nervous system and is considered as one of the useful neuron subpopulation markers. In the present study we reported that SCGN specifically labelled a particular neuronal cluster in the brainstem of the mice and rats. The comparison of the SCGN immunostaining with the choline acetyltransferase immunostaining and acetylcholinesterase staining clearly indicated that the particular cluster of SCGN positive neurons corresponded to the microcellular tegmental nucleus (MiTg) and the ventral portion of the cuneiform nucleus (CnF), both of which are components of the isthmus. The analyses in mice indicated that SCGN positive neurons in the MiTg and CnF were homogeneous in size and shape, appearing to compose a single complex: their somata were small comparing with the adjacent cholinergic neurons in the pedunculotegmantal nucleus, 10.5 vs 16.0â¯Î¼m in diameter, and extended 2-3 slender smooth processes. SCGN might be one of significant markers to reconsider the delineations of the structures of the mouse, and presumably rat, brainstem.
Keywords
Cy5CNFNeuNHDBLFPFasciculus retroflexusLDTgvlPAGDLGPBGLPBCy3indodicarbocyanineIndocarbocyaninePTGIndusium griseummedial terminal nucleus of the accessory optic tractXSCPmiTGSCGNmicrocellular tegmental nucleusDAPIPBSGFPSCPMCPFITCAPTMLFCPU4′, 6-diamidino-2-phenylindoleBSADllaqueductbovine serum albuminAChEBrain atlassuperior cerebellar peduncleAcetylcholinesteraseImmunohistochemistryphosphate bufferlateral ventricleSuperior colliculusMiddle cerebellar peduncleSecretagoginFacial nerveMedial longitudinal fasciculusfluorescein isothiocyanatedecussation of the superior cerebellar peduncleLateral lemniscusmedial lemniscusPhosphate-buffered salineCerebellumoculomotor nucleusParabigeminal nucleustrochlear nucleuscuneiform nucleusmotor trigeminal nucleusnucleus of the horizontal limb of the diagonal banddorsal lateral geniculate nucleuslateral parabrachial nucleusdorsal nucleus of the lateral lemniscusanterior pretectal nucleuslaterodorsal tegmental nucleusfacial nucleusanteroventral thalamic nucleusHistochemistryHippocampusCaudate putamenParvalbumingreen fluorescent proteinolfactory bulbChATCalretinincalbindincholine acetyltransferaseInferior colliculus
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Authors
Toshio Kosaka, Katsuko Kosaka,