Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8871390 | Marine Pollution Bulletin | 2018 | 7 Pages |
Abstract
This study investigated the anti-estrogenic effects of chronic exposure to a new marine pollutant, semicarbazide (SMC; 1, 10, and 100 μg/L), in female Paralichthys olivaceus, as well as the associated mechanism. After 130 days of exposure, plasma 17β-estradiol and testosterone concentrations, and hepatic estrogen receptors, vitellogenin, and choriogenin mRNA levels decreased significantly in SMC-exposed groups. Moreover, down-regulation of genes in the hypothalamic-pituitary-gonadal axis, including gonadotropin-releasing hormone, gonadotropic hormones and their receptors, the steroidogenic acute regulatory protein, 17α-hydroxylase, 17β-hydroxysteroid dehydrogenase, and cytochrome P450 19A, was observed after SMC exposure. Furthermore, the kisspeptin/g protein-coupled receptor 54 (kiss/gpr54) system and gamma-aminobutyric acid-ergic (GABAergic) system were also affected by SMC: SMC significantly down-regulated mRNA expression of kiss2, gpr54, and the GABA synthesis enzyme gad67. Our results demonstrated for the first time that environmentally relevant concentrations of SMC exerted anti-estrogenicity in female flounders, providing theoretical support for ecological risk assessments of SMC in marine environments.
Keywords
LHRCYP173β-HSD17β-HSDFSHRDPH3β-Hydroxysteroid dehydrogenase17β-Hydroxysteroid dehydrogenaseCYP11A1SMCGPR54FSHβcyp19agonadotropic hormonesGnRHlhβcytochrome P450 11A1sGnRHMS-222ChoriogeninERSGADVitellogenin17α-hydroxylase17β-estradiolEDCsglutamic acid decarboxylaseELSgamma-aminobutyric acidKISSELISAEnzyme-linked immunosorbent assaytestosteronedays post hatchingStarsemicarbazideJapanese flounderHPG axisHypothalamic-pituitary-gonadal axisearly life stagesEndocrine Disrupting ChemicalsGonadotropin-releasing hormonefollicle-stimulating hormoneluteinizing hormoneFSHvtgSteroidogenic acute regulatory proteinchgKisspeptinGABAestrogen receptorsfollicle-stimulating hormone receptorLuteinizing hormone receptor
Related Topics
Physical Sciences and Engineering
Earth and Planetary Sciences
Oceanography
Authors
Zonghao Yue, Miao Yu, Haifeng Zhao, Jun Wang, Xiaona Zhang, Hua Tian, Wei Wang, Shaoguo Ru,