Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8872163 | Marine Pollution Bulletin | 2017 | 11 Pages |
Abstract
ATP-binding cassette (ABC) transporters participate in transporting various substances, including xenobiotics, in or out of cells. However, their genetic information and function in ciliates remain still unclear. In this study, we sequenced and characterized two ABC transporter genes (EcABCB and EcABCC), and investigated the effect of cadmium (Cd) and benzo[a]pyrene (B[a]P) on their function and gene expression, using efflux assay and real-time reverse transcription-polymerase chain reaction (qRT-PCR), respectively, in the marine ciliate, Euplotes crassus. Sequencing analysis and efflux assay showed that EcABCB and EcABCC are typical ABC transporters, possessing conserved function. Exposure to Cd (â¥Â 5 mg/L) and B[a]P (â¥Â 50.5 μg/L) enhanced accumulation of a substrate. A significant increase in the expression of EcABCB and EcABC mRNA was observed at lower concentration in response to Cd and B[a]P. Our findings indicate that Cd and B[a]P could inhibit the efflux function of ABC transporters, leading to cellular toxicity in the ciliate.
Related Topics
Physical Sciences and Engineering
Earth and Planetary Sciences
Oceanography
Authors
Hokyun Kim, Bora Yim, Jisoo Kim, Haeyeon Kim, Young-Mi Lee,