Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8872272 | Marine Pollution Bulletin | 2017 | 4 Pages |
Abstract
We investigated the effect of combined exposure to nodavirus infection and TBT on medaka (Oryzias latipes). Medaka larvae were infected by immersion in medium containing nodavirus at titers of 102.5, 103.5, or 104.5 TCID50/mL. Infected fish then were exposed to TBT at 0, 0.17, 0.52, 1.6, or 4.7 μg/L. Of the 12 groups exposed to both stressors, the mortalities of 6 (102.5 TCID50/mL + 0.52, 1.6, or 4.7 μg/L, 103.5 TCID50/mL + 4.7 μg/L and 104.5 TCID50/mL + 1.6 or 4.7 μg/L) were significantly higher than that of each TBT control. Specifically, mortality was 46 ± 5.5% in the group exposed to both 102.5 TCID50/mL virus and 0.52 μg/L TBT, which represent the lowest observed effective dose and concentration, respectively, among the 6 groups with increased mortalities. Our results suggest that combined exposure to both stressors suppresses antiviral mechanisms in the fish, thus increasing mortality.
Related Topics
Physical Sciences and Engineering
Earth and Planetary Sciences
Oceanography
Authors
Shin-Ichi Kitamura, Masaki Akizuki, Jun-Young Song, Kei Nakayama,