| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 8942675 | Journal of Chromatography B | 2018 | 27 Pages |
Abstract
This work provides examples of milder conditions (65â¯Â°C and 0.03% TFAâ¯+â¯0.07% FA) being successfully employed in the RPLC(-MS) analysis of mAb subunits. A suitable stationary phase was selected, and milder conditions allow an improved MS sensitivity, while maintaining comparable elution profiles. Moreover, the use of milder conditions may reduce the risk of artefact peaks.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Balázs Bobály, Valentina D'Atri, Matthew Lauber, Alain Beck, Davy Guillarme, Szabolcs Fekete,