Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8947787 | Biosensors and Bioelectronics | 2018 | 23 Pages |
Abstract
An ultrasensitive label-free photoelectrochemical (PEC) immunosensor with high visible-light activity was developed for quantitative detection of amyloid β-protein (Aβ) by cross-linking anti-Aβ antibody onto the Ag2S sensitized SnO2/SnS2 nanocomposites. Specifically, SnO2 with flower-like porous nanostructure was innovatively applied in PEC immunosensor as a basal material. It could form a heterostructure with SnS2, which brought about the sensitization of SnO2 and enhanced the separation of photogenerated electrons and holes. Moreover, Ag2S was in-situ growth on the surface of SnO2/SnS2, which further enhanced the photocurrent response significantly. Therefore, SnO2/SnS2/Ag2S could form stepwise band-edge structure, which benefited the light harvesting and provided a good foundation for sensor construction and detection. Under optimal conditions, the PEC immunosensor was used to detect the content of Aβ and exhibited a wide linear concentration range from 0.5â¯pgâ¯mLâ1 to 100â¯ngâ¯mLâ1, with low limit of detection (0.17â¯pgâ¯mLâ1) and limit of quantification (0.56â¯pgâ¯mLâ1). Additionally, the designed PEC immunosensor exhibited good reproducibility, specificity, and stability which may find potential applications in the biosensor, biomedicine, clinical diagnosis, photocatalysis and other related fields.
Related Topics
Physical Sciences and Engineering
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Analytical Chemistry
Authors
Yaoguang Wang, Dawei Fan, Guanhui Zhao, Jinhui Feng, Dong Wei, Nuo Zhang, Wei Cao, Bin Du, Qin Wei,