Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8959288 | Saudi Journal of Biological Sciences | 2018 | 6 Pages |
Abstract
Plantago species are used as traditional medicine in Asian and Europe. Polysaccharide isolated from the seeds of Plantago asiatica L. could stimulate maturation transformation of bone-marrow derived dendritic cells (DCs). We found that blocking p38, ERK1/2 and JNK MAPK signal transduction could significantly decreased the PLP-2 induced expression of MHC II, CD86 surface molecules on DCs. Blocking p38 and JNK signal also significantly inhibited the cytokine secretion of TNF-α and IL-12p70 as well, while blocking ERK1/2 signal only decreased the secretion of TNF-α. Meanwhile, DCs in the three MAPK signal-blocking groups showed dramatically attenuated effects on stimulating proliferation of T lymphocytes. Similarly, blocking signal transduction of NF-κB pathway also significantly impaired the phenotypic and functional maturation development of DCs induced by PLP-2. These data suggest that MAPK and NF-κB pathway mediates the PLP-induced maturation on DCs. Especially, among the three MAPK pathways, activation of JNK signal transduction is the most important for DCs development after PLP-2 incubation. And PLP-2 may activate the MAPK and NF-κB pathway by triggering toll-like receptor 4 on DCs.
Keywords
NF-κBTNFJnkPAMPsERKc-Jun amino-terminal kinasesDCsPRRPLPPDTCMAPKpathogen-associated molecular patternsinterleukincluster of differentiationpyrrolidine dithiocarbamateDendritic cellsextracellular signal regulated kinasestumor necrosis factornuclear factor κBmitogen-activated protein kinasepattern recognition receptors
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Authors
LeMing Jiang, DanFei Huang, ShaoPing Nie, MingYong Xie,