Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8962008 | Biologicals | 2018 | 8 Pages |
Abstract
All FBS lots and one lot of bovine-derived trypsin were PCR-positive for the presence of BVDV genome; in contrast all vaccine lots and the other samples were negative. qRT-PCR based virus replication assay and immunofluorescence-based infection assay detected no infectious BVDV in the PCR-positive samples. Complete BVDV genomes were generated from FBS samples by deep sequencing, and all were BVDV type 1. These data confirmed that BVDV nucleic acid may be present in bovine-derived raw materials, but no infectious virus or genomic RNA was detected in the final vaccine products.
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Authors
Majid Laassri, Edward T. Mee, Sarah M. Connaughton, Hasmik Manukyan, Marion Gruber, Carmen Rodriguez-Hernandez, Philip D. Minor, Silke Schepelmann, Konstantin Chumakov, David J. Wood,