Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
8962667 | Journal of Oral Biosciences | 2018 | 6 Pages |
Abstract
Latent TGF-β1 produced and secreted from secretory-stage ameloblasts is activated by MMP20, and the activated TGF-β1 maintains its activity by combining with amelogenin cleavage products processed by the same protease. TGF-β1 moves through the aqueous phase with the water-soluble 13â¯kDa amelogenin and binds to its receptor on the ameloblast surface, thereby inducing autocrine signaling. Once the ameloblasts differentiate and enter the maturation stage, TGF-β1 is degraded by KLK4, which is produced and secreted by maturation-stage ameloblasts, and loses its activity.
Keywords
MMPTGF-βTGFBR1RT-PCRqPCRALPAlkaline phosphataseAutocrinesodium dodecyl sulfate-polyacrylamide gel electrophoresisSDS-PAGETransforming Growth Factor Betatransforming growth factor-betaEnzyme-linked immunosorbent assayELISAToothmatrix metalloproteinaseBMPEnamelreverse transcription-polymerase chain reactionquantitative polymerase chain reactionProteinbone morphogenic proteinhigh performance liquid chromatographyHPLC
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Authors
Saeko Kobayashi, Yasuo Yamakoshi, Yoshinobu Asada,