Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9007849 | International Immunopharmacology | 2005 | 4 Pages |
Abstract
Induction of anesthesia by inhalation of isoflurane is a frequently used procedure in models of immunological diseases. Here we investigated effects of a brief exposure to isoflurane on cytokine production by endotoxemic rats. Anesthesia was either performed by pentobarbital/fentanyl without or accompanied by a 50-s inhalation pretreatment with isoflurane. After 4 h of endotoxemia, plasma levels of TNFα, IL-1β, and RANTES were determined. Isoflurane significantly inhibited plasma levels of TNFα and IL-1β by 69.3% and 61.8%, respectively. Levels of RANTES were similarly reduced by 43.1% (n.s.). Moreover, isoflurane significantly attenuated basal nitrite production by alveolar macrophages isolated from endotoxemic rats (by 59.4%). Thus, we confirm anti-inflammatory properties of isoflurane and beyond that clearly demonstrate that even a short exposure (< 1 min) can profoundly affect pro-inflammatory parameters in experimental endotoxemia. These unforeseen observations suggest that short-term inhalation of isoflurane for induction of anesthesia may be an unsuitable procedure particularly in animal models of acute inflammation.
Keywords
Related Topics
Life Sciences
Immunology and Microbiology
Immunology
Authors
Christian Hofstetter, Michael Flondor, Kim A. Boost, Patricia Koehler, Markus Bosmann, Josef Pfeilschifter, Bernhard Zwissler, Heiko Mühl,