| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9030065 | Environmental Toxicology and Pharmacology | 2005 | 4 Pages |
Abstract
We report preliminary results from a proteomic search for rat brain protein targets adducted by organophosphorous pesticides. Azamethaphos, chlorfenvinphos, diazinon, malathion and chlorpyrifos oxons (in rat brain homogenates) or pirimiphos-methyl (after systemic treatment) were tested at levels producing no more than 30% inhibition of brain acetylcholinesterase. Loss of reactivity with tritiated diisopropylflurophosphate was taken as proof of adduction by the test organophosphate. In addition to acetylcholinesterase other, previously unrecognised, adducted proteins were detected in total brain protein extracts at 30, 32, 41, 71 and 83Â kDa. Azamethiphos adducted all but the 30 and 32Â kDa bands, but chlorpyrifos only acetylcholinesterase.
Related Topics
Life Sciences
Environmental Science
Health, Toxicology and Mutagenesis
Authors
Andrea Murray, Alexandra J. Rathbone, David E. Ray,