Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9034146 | Reproductive Toxicology | 2005 | 10 Pages |
Abstract
Pregnancy outcomes were evaluated following uterine transfer of murine preimplantation embryos exposed in vitro to the estrogenic pesticide o,pâ²-dichlorodiphenyltrichloroethane (o,pâ²-DDT). Single-cell embryos were incubated 72 h in medium droplets containing 0.1% ethanol (control) or 0.1 μg/ml o,pâ²-DDT (pesticide). Morula and preblastocyst embryos were transferred in groups of eight to right uterine horns of pseudopregnant mice (n = 111) and pups (n = 132) were evaluated at Caesarean-section (C-section). In vitro exposure to o,pâ²-DDT reduced development to morula (P < 0.001) and modestly increased blastomere apoptosis (P = 0.05). However, treatment differences were not detected for implantation rates (35% versus 39%; P = 0.64), pup numbers per dam (2.3 versus 1.9; P = 0.36), transfer efficiencies (16% versus 14%; P = 0.53), fetal weights (1.56 g versus 1.57 g; P = 0.91), skeletal abnormalities (55% versus 66%; P = 0.47), or male ratios (54.8% versus 53.8%; P = 1.0). In vitro exposure of preimplantation embryos to 0.1 μg/ml o,pâ²-DDT for 72 h resulted in no measurable effects on subsequent implantation or pup characteristics at C-section.
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Authors
Anne R. Greenlee, Tammy M. Ellis, Richard L. Berg, Michael D. Mercieca,