Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9037229 | Toxicology Letters | 2005 | 8 Pages |
Abstract
Myristicin, 1-allyl-3,4-methylenedioxy-5-methoxybenzene, is a naturally occurring alkenylbenzene compound found in the nutmeg. The present study was conducted to assess the cytotoxic and apoptotic effects of myristicin on the human neuroblastoma SK-N-SH cells. We found that a dose-dependent reduction in cell viability occurs at myristicin concentration â¥0.5 mM in SK-N-SH cells. Apoptotic cell death was confirmed using DNA fragmentation, terminal deoxyribonucelotidyl transferase-mediated dUTP nick end labeling and by 4,6-diamidino-2-phenylindole staining. Microscopy was used to observe apoptotic cell morphology. Western blotting was used to investigate the protein expression of known apoptotic mediators including cytochrome c, caspase-3, and PARP. The apoptosis triggered by myristicin was accompanied by an accumulation of cytochrome c and by the activation of caspase-3. The results obtained suggest that myristicin induces cytotoxicity in human neuroblastoma SK-N-SH cells by an apoptotic mechanism. This myristicin-induced apoptosis provides further insight of the molecular mechanisms of myristicin toxicity.
Related Topics
Life Sciences
Environmental Science
Health, Toxicology and Mutagenesis
Authors
Bo Kyung Lee, Jae Hee Kim, Ji Wook Jung, Ji Woong Choi, Eui Sik Han, Sun Hee Lee, Kwang Ho Ko, Jong Hoon Ryu,