Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9037472 | Toxicology Letters | 2005 | 13 Pages |
Abstract
CD34+ progenitor derived DC were exposed to nine well-known allergens (one weak, three moderate and five strong allergens) and two irritants. Surface marker expression (CD86, CD83 and HLA-DR) and cytokine production (IL-6, IL-12 and TNF-α) were analyzed after 24 h exposure to these chemicals. All allergens tested induced a significant increase in at least one of the DC surface markers. In contrast, none of the irritants tested were able to significantly upregulate membrane marker expression in exposed DC. The level of upregulation of CD86, CD83 and HLA-DR was dependent on the nature and concentration of the chemical, but not on the classification of the allergen. Changes in cytokine production (IL-6, IL-12 and TNF-α) were not consistently related to exposure to an allergen. Based on these results, we conclude that the in vitro test using CD34-DC has the capacity to distinguish between allergens and irritants based on altered phenotypic characteristics.
Keywords
dinitrobenzenesulfonic acidEugDNFBMo-DCDNBSTNBSIL-12OXADNCBCD34+ progenitor cellsSDSIL-62,4,6-Trinitrobenzene sulfonic acidAllergensEugenolOxazoloneinterleukin-12interleukin-6Benzalkonium chlorideCinPotassium dichromatedinitrofluorobenzeneDinitrochlorobenzenesodium dodecyl sulfateDendritic cellsCytokinesstimulation indexRecombinant human tumor necrosis factor-αNickel
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Authors
Ann C.A. De Smedt, Rosette L. Van Den Heuvel, Vigor F.I. Van Tendeloo, Zwi N. Berneman, Greet E.R. Schoeters,