Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9038821 | Toxicology in Vitro | 2005 | 8 Pages |
Abstract
TD, produced by tire wear, is a significant constituent of PM10 in urban areas where traffic related emissions are predominant. TD contains a lot of chemicals which can affect human respiratory system and it has received little attention until now, even the toxicity of PM has been extensively documented. A549 cells, a human alveolar lung cells, were exposed for 24, 48, 72 h to 10, 50, 60, 75 μg/ml of TD organic extract. MTT and Trypan Blue assays were used to evaluate cytotoxicity and Comet Assay to evidence DNA damage. TD extracts induced a dose-dependent increase in cell mortality and DNA damage. A significant toxicity was observed when cells were exposed to 60 μg/ml for 72 h. Moreover cell morphology observed at ultra structural level, was severely affected at the highest dose.
Related Topics
Life Sciences
Environmental Science
Health, Toxicology and Mutagenesis
Authors
Maurizio Gualtieri, Laura Rigamonti, Valentina Galeotti, Marina Camatini,