| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9103465 | Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) | 2005 | 9 Pages |
Abstract
Age-related changes in adenyl purine release from rat arteries and endothelial cell (EC) plasma membrane (PM) fluidity were studied. High performance liquid chromatography-fluorescence revealed that aging significantly decreased the release of adenyl purines. Pyrene-excimer spectroscopy disclosed that EC PM fluidity of aged rats decreased more significantly than that of young rats. An increase in cholesterol content and a decrease in the unsaturation index (USI) of fatty acids in cholesterol-enriched ECs reduced PM fluidity and 5â²-nucleotidase (5â²-ND) activity (measured by coupled assay of adenosine deaminase and glutamate dehydrogenase). Moreover, a decrease in cholesterol content and an increase in the USI of fatty acyl chains of the PM in docosahexaenoic acid-enriched ECs concurrently increased enzyme activity and extracellular adenosine. Therefore, decreases in PM fluidity, observed with age-dependent increased cholesterol and decreased USI, induce a decrease in 5â²-ND activity, decrease extracellular adenosine levels, and might relate to hypertension in aged rats.
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Authors
Michio Hashimoto, Shahdat Hossain, Yoko Tanabe, Osamu Shido,