Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9104597 | Bone | 2005 | 5 Pages |
Abstract
Seven of the eight applied fluorochromes could be resolved using spectral image examination. With BAPTA, we present a new fluorochrome suitable for bone labeling. Due to the superior sensitivity of the spectral image acquisition, the thickness of the bone sections could be reduced so that 5 μm thick sections could be analyzed. Spectral decomposition and subsequent linear unmixing allows depiction of each individual fluorochrome without interference of any other, enabling a reliable and superior morphometric analysis of labeled regions.
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Authors
Christoph Pautke, Stephan Vogt, Thomas Tischer, Gabriele Wexel, Herbert Deppe, Stefan Milz, Matthias Schieker, Andreas Kolk,