Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9110906 | Cytokine | 2005 | 7 Pages |
Abstract
Interleukin (IL)-12 is an inducer of differentiation of T helper (Th) cells towards Th1, whereas IL-10 is mainly an anti-inflammatory cytokine inhibiting Th1 functions. Single nucleotide polymorphisms (SNPs) in the regulatory sequences of genes are presumed to be associated with the differential production of cytokines. One IL12B 3â²untranslated region (UTR) and five of IL10 gene promoter region SNPs were screened in 152 individuals by genotyping. IL-10 and IL-12 secretion of lipopolysaccharide (LPS), purified protein derivative (PPD) and Staphylococcus aureus Cowan strain I (SAC) stimulated peripheral blood mononuclear cells (PBMCs) was determined. The frequencies of the less frequent IL12B +16974 C, IL10 â2763 A â3575 A, â1082 G, â819 T and â592 A alleles were 27.4, 32.2, 25.9, 14.8, 9.3 and 8.6%, respectively. Individuals CC homozygous at IL12B 3â²UTR had significantly higher IL-12 secretion levels from LPS and PPD stimulated PBMCs than AC heterozygotes (PÂ =Â 0.03 and PÂ =Â 0.02) or AA homozygotes (PÂ =Â 0.02 and PÂ =Â 0.05, respectively). IL10 â2763 and â3575 SNPs did not show any effect on in vitro secretion levels, whereas the association of proximal promoter â1082 SNP with IL-10 production is confirmed. IL10 proximal and distal promoter SNP distribution with estimated haplotype variations has implicated considerable similarities with the Caucasian populations in Turkey.
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Authors
Vuslat Yılmaz, Sibel P. Yentür, Güher Saruhan-Direskeneli,