Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9121253 | FEMS Microbiology Letters | 2005 | 6 Pages |
Abstract
Quercetinase is produced by various filamentous fungi when grown on rutin as sole carbon and energy source. We investigated on the effect of 10 phenolics and two sugars, structurally related to substrates and products of the rutin catabolic pathway, on the induction of a quercetinase activity in Penicillium olsonii. Neither the sugars (glucose and rhamnose, two constituents of rutin), nor phenolics such as protocatechuic acid, salicylic acid, 4-hydroxy-benzoic acid and phloroglucinol were inducers. Rutin (maximum activity 150Â nmol/min/mL after 5 days), quercetin (70Â nmol/min/mL, 3 days), phloroglucinol carboxylic acid (60Â nmol/min/mL, 3 days), 2-protocatechuoylphloroglucinolcarboxylic acid (50Â nmol/min/mL, 5 days), 2,6-dihydroxy-carboxylic acid (90Â nmol/min/mL, 7 days) and 2,4-dihydroxy-carboxylic acid (30Â nmol/min/mL, 7 days) were demonstrated to be quercetinase inducers. We propose that rutin, quercetin and 2-protocatechuoyl-phloroglucinol carboxylic acid, the product of the reaction catalysed by quercetinase, act as inducers after their catabolic transformation in phloroglucinol carboxylic acid.
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Authors
Sylvain Tranchimand, Thierry Tron, Christian Gaudin, Gilles Iacazio,