| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9121285 | FEMS Microbiology Letters | 2005 | 8 Pages |
Abstract
Enterohaemorrhagic Escherichia coli (EHEC) contains a 36-kb pathogenicity island termed the locus of enterocyte effacement (LEE), which encodes a type III secretion system (TTSS) and virulence proteins. In this paper, we show that the O157:H7 Sakai clpPX mutant strongly impaired the secretion of virulence proteins by TTSS and repressed transcription from all the LEE promoters. The rpoS mutation in O157:H7 Sakai enhanced the transcription from all the LEE promoters and the secretion of virulence proteins, and it could partially suppress the defects of the clpPX mutation. These data indicate that the O157:H7 Sakai ClpXP protease is a positive regulator for LEE expression and that this regulation occurs by two pathways: the ÏS-dependent and -independent pathways.
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Authors
Toshifumi Tomoyasu, Akiko Takaya, Yutaka Handa, Kiyonobu Karata, Tomoko Yamamoto,