| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9121305 | FEMS Microbiology Letters | 2005 | 7 Pages |
Abstract
Loop-mediated isothermal amplification (LAMP) assay detected Salmonella within 60Â min. The 220 strains of 39 serotypes of Salmonella subsp. enterica and 7 strains of Salmonella enterica subsp. arizonae were amplified, but not 62 strains of 23 bacterial species other than Salmonella. The sensitivity of the LAMP assay was found to be >2.2Â cfu/test tube using nine serotypes. The specificity was similar to that of a PCR assay, but the sensitivity of LAMP was greater. Both fluorescence and turbidity were able to detect the products in the LAMP assay. S. enteritidis in a liquid egg sample artificially inoculated with the organism was detected by the LAMP assay at 2.8Â cfu/test tube, although negative by PCR assay. These results indicate that the LAMP assay is a rapid, specific and sensitive detection method for Salmonella.
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Authors
Yukiko Hara-Kudo, Manabu Yoshino, Tadashi Kojima, Masanari Ikedo,