Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9121315 | FEMS Microbiology Letters | 2005 | 9 Pages |
Abstract
An Escherichia coli-Laribacter hongkongensis shuttle vector (pPW380) was constructed by ligating the 4701-bp EcoRI digested fragment of pHLHK8 to EcoRI digested pBK-CMV. An E. coli-L. hongkongensis inducible expression shuttle vector was further constructed by ligating a 2105-bp fragment that contains the tetracycline repressor and tetracycline-inducible promoter region of pALC2084 to the 8897-bp fragment of pPW380, deletion of the green fluorescent protein gene, and insertion of a multiple cloning site. This inducible expression system was able to express two commonly used reporter genes, the green fluorescent protein gene and the glutathione S-transferase gene, efficiently in E. coli and L. hongkongensis.
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Authors
Patrick C.Y. Woo, Shirley S.L. Ma, Jade L.L. Teng, Maria W.S. Li, Richard Y.T. Kao, Susanna K.P. Lau, Kwok-yung Yuen,