Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9121419 | FEMS Microbiology Letters | 2005 | 5 Pages |
Abstract
We report the identification and nucleotide sequence analysis of a cryptic plasmid pMIDG2830 from the Gram-negative bacterium Neisseria flavescens. The largest open reading frame encodes a protein similar to the replication protein, RepA, found in pAB49 from Acinetobacter baumannii and pNI10 from Pseudomonas. Modified by the incorporation of a kanamycin resistance cassette, the plasmid can be stably maintained in Escherichia coli and Neisseria meningitidis, and can be used as a shuttle plasmid in meningococcal research.
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Authors
ClÃona A. O' Dwyer, Paul R. Langford, J. Simon Kroll,