| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9121667 | FEMS Microbiology Letters | 2005 | 6 Pages |
Abstract
Intraspecies variation of Chromobacterium violaceum was examined by comparative sequence - and by restriction fragment length polymorphism analysis of the recombinase A gene (recA-PCR-RFLP). Primers deduced from the known recA gene sequence of the type strain C. violaceum ATCC 12472T allowed the specific amplification of a 1040Â bp recA fragment from each of the 13 C. violaceum strains investigated, whereas other closely related organisms tested negative. HindII-PstI-recA RFLP analysis generated from 13 representative C. violaceum strains enabled us to identify at least three different genospecies. In conclusion, analysis of the recA gene provides a rapid and robust nucleotide sequence-based approach to specifically identify and classify C. violaceum on genospecies level.
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Authors
Holger Christian Scholz, Angela Witte, Herbert Tomaso, Sascha Al Dahouk, Heinrich Neubauer,