Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9121873 | FEMS Microbiology Letters | 2005 | 7 Pages |
Abstract
Exposure to high pressure induces germination in spores of Bacillus subtilis. To investigate the mechanisms of this process and to compare the pressure and nutrient induced germination pathways, a random transposon knock-out library of B. subtilis was constructed and screened for clones with a compromised pressure induced germination at 100Â MPa. Two mutants were isolated and their transposon insertion was mapped to gerAC and ykvU respectively. While GerAC is required for production of the l-alanine receptor which has been implicated in pressure-induced germination before, YkvU is shown here to be a novel germination determinant in B. subtilis, affecting germination by high (100Â MPa) and very high (600Â MPa) pressure, by nutrients and by dodecylamine, but not by Ca2+-dipicolinic acid.
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Authors
Abram Aertsen, Isabelle Van Opstal, Suzy C. Vanmuysen, Elke Y. Wuytack, Chris W. Michiels,