Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9121916 | FEMS Microbiology Letters | 2005 | 8 Pages |
Abstract
The DNA sequence of the gyrB gene is a suitable phylogenetic marker for bacterial systematics. In this study, the diversity of Aeromonas spp. present in environmental samples was assessed by a PCR combined with DGGE approach. PCR primers targeting the gyrB gene of aeromonads were designed and the resulting amplicons were analyzed by DGGE. The gyrB-DGGE analysis was evaluated with Aeromonas isolates and reference strains allowing discrimination of the majority of strains. The gyrB-DGGE analysis is a powerful tool to monitor the presence and evaluate the diversity of aeromonads in complex samples, as is the case of water from a wastewater treatment plant.
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Authors
Marta Tacão, Alexandra Moura, Artur Alves, Isabel Henriques, Maria José Saavedra, António Correia,