Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9121982 | FEMS Microbiology Letters | 2005 | 8 Pages |
Abstract
Endoglucanase and xylanase activities of three rumen protozoa, Polyplastron multivesiculatum, Eudiplodinium maggii, and Entodinium sp. were compared qualitatively by zymograms and quantitatively by measuring specific activities against different polysaccharides. A set of carboxymethylcellulases and xylanases was produced by the large ciliates whereas no band of activity was observed for Entodinium sp. in zymograms. Specific activity of endoglucanases from P. multivesiculatum (1.3 μmol mg protâ1 minâ1) was twice that of E. maggii, whereas xylanase specific activity (4.5 μmol mg protâ1 minâ1) was only half. Very weak activities were observed for Entodinium sp. A new xylanase gene, xyn11D, from P. multivesiculatum was reported and its gene product compared to 33 other family 11 xylanases. Phylogenetic analysis showed that xylanase sequences from rumen protozoa are closely related to those of bacteria.
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Authors
Christel Béra-Maillet, Estelle Devillard, Magalie Cezette, Jean-Pierre Jouany, Evelyne Forano,