Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9121997 | FEMS Microbiology Letters | 2005 | 7 Pages |
Abstract
Classification and differentiation of Bacillus anthracis isolates by genetic markers play an important role in anthrax research. We used a PCR based method - Random Amplification of Polymorphic DNA (RAPD) - to identify genetic markers in B. anthracis strains. Twenty-five differential genetic markers were identified which divided the strains into five different groups. Three selected RAPD-markers were cloned and sequenced. The five RAPD-derived genotypes could be defined by integration of these three markers. This system offers a simple non-expensive method to classify B. anthracis strains in laboratories involved in the research of this bacterium.
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Authors
Haim Levy, Morly Fisher, Naomi Ariel, Zeev Altboum, David Kobiler,