| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9122094 | FEMS Microbiology Letters | 2005 | 7 Pages |
Abstract
Multidrug efflux pumps contribute to multiple antibiotic resistance in Pseudomonas aeruginosa. Pump expression usually has been quantified by Western blotting. Quantitative real-time polymerase chain reaction has been developed to measure mRNA expression for genes of interest. Whether this method correlates with pump protein quantities is unclear. We devised a real-time PCR for mRNA expression of MexAB-OprM and MexXY-OprM multidrug efflux pumps. In laboratory strains differing in MexB and MexY expression and in several clinical isolates, protein and mRNA expression correlated well. Quantitative real-time PCR should be a useful alternative in quantitating expression of multidrug efflux pumps by P. aeruginosa isolates in clinical laboratories.
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Authors
Kazuhiko Yoneda, Hiroki Chikumi, Takeshi Murata, Naomasa Gotoh, Hiroyuki Yamamoto, Hiromitsu Fujiwara, Takeshi Nishino, Eiji Shimizu,