Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9122136 | FEMS Microbiology Letters | 2005 | 7 Pages |
Abstract
Aeromonas sobria hemolysin (ASH) is one of the major virulence factors produced by A. sobria, a human pathogen that causes diarrhea. We investigated the effects of ASH on Clâ transport in human colonic epithelial cells. ASH increased short-circuit currents (Isc) and 125I efflux from Caco-2 cells, indicating ASH activate Clâ secretion. Additions of inhibitors of cyclic AMP dependent Clâ channels, glybenclamide and NPPB suppressed the Isc and 125I efflux increases induced by ASH. And ASH increased the intracellular cyclic AMP concentration. Moreover, ASH stimulated fluid accumulation in the iliac loop test, and glybenclamide and NPPB suppressed this fluid accumulation. Thus, cAMP-dependent Clâ secretory pathway could be related with diarrhea induced by A. sobria.
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Authors
Naomi Tanoue, Akira Takahashi, Keinosuke Okamoto, Yoshio Fujii, Yutaka Taketani, Nagakatsu Harada, Masayuki Nakano, Yutaka Nakaya,