Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9126933 | Gene | 2005 | 10 Pages |
Abstract
After sexual reproduction in ciliated protozoa a new macronucleus differentiates from a micronuclear derivative. In the course of macronuclear development dramatic DNA- and chromatin reorganisation processes occur, which include splicing of DNA sequences such as IES (internal eliminated sequences) and transposon-like elements during formation of polytene chromosomes, degradation of the polytene chromosomes and specific elimination of micronuclear-specific DNA, de novo addition of telomeres and specific amplification of DNA sequences. In order to understand the molecular basis of this nuclear differentiation process, analysis of developmentally regulated genes seems to be a necessary prerequisite. We performed a microarray analysis to identify genes differentially expressed during macronuclear differentiation. 467 sequences from cDNA libraries were identified as possible candidates from which 384 sequences were further characterised by sequence analysis. These sequences were identified, if possible, by DNA and protein BLAST analysis. Expression of one of these sequences was silenced by RNAi and a preliminary functional analysis performed. Results presented in this study provide the basis for a functional characterisation of genes differentially expressed during this nuclear differentiation process.
Keywords
HmgNuclear differentiationRF-CPCNARRMRecAMDPIESRNA interferenceSmall interfering RNARNA recognition motifRNAisiRNAProliferating Cell Nuclear AntigenBasic Local Alignment Search ToolE-valueExpectation valueGene expression patternBlastinternal eliminated sequencesVegetative cellsciliatescDNA microarrayhigh mobility group
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Genetics
Authors
Adrian G. Paschka, Volker Horejschi, Franziska Jönsson, Antje Lindecke, Gero Weier, Christian Kaltschmidt, Hans J. Lipps,