Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9126935 | Gene | 2005 | 12 Pages |
Abstract
The ADAMTS (A Disintegrin and metalloproteinase with thrombospondin-1 type repeats) family of enzymes have been implicated in turnover of extracellular matrix. We previously showed that levels of ADAMTS6 mRNA in ARPE-19 cells were markedly increased following treatment with tumour necrosis factor alpha (TNFα). This study shows that the ADAMTS6 transcript contains unusually large untranslated regions (UTRs) at both the 5â² and 3â²end. The 5â²UTR contains 11 AUG codons upstream of the predicted ADAMTS6 start codon and potently inhibits translation of a downstream reporter gene. However some translation can be restored by truncating the 5â²UTR from the 5â²end. The 5â²UTR was tested for internal ribosome entry site activity using a bicistronic luciferase reporter plasmid, but none was detected. Using the 5â² and 3â²UTR sequences to screen the GenBank database we identified a full length ADAMTS6 cDNA of 7262 bp. This transcript is alternatively spliced at the 3â²end of the open reading frame (ORF), resulting in an extended ORF containing 3 additional tsp-1 type repeats. Quantitative RT-PCR showed that the long and short form of the ADAMTS6 ORF are co-expressed in ARPE-19 cells, but the relative levels of the two forms is modulated by TNFα. The region of the transcript encoding the catalytic domain also contains several notable differences compared to the previously published ADAMTS6 cDNA sequence, including a redefinition of the predicted active site motif.
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Authors
Debra J. Bevitt, Zheng Li, Jenny L. Lindrop, Michael D. Barker, Michael P. Clarke, Norman McKie,