Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9127037 | Gene | 2005 | 12 Pages |
Abstract
The availability of genomic clones representative of the T cell receptor gamma (TRG1@ and TRG2@) ovine loci enabled us to compare the germline genomic organization and nucleotide diversity of joining (J) segments and reconstruct their evolutionary history by phylogenetic analysis of cattle, sheep and human expressed sequences. Expression profiling (RT-PCR data) in fetus and adult indicated that only the ovine J genes in which two or more of the key sequence features, such as recombination signal sequences (RSS), 3â² splice sites, and core sequences, are missing or severely altered fail to be transcribed. Comparative genomic examination of the two human with the six sheep germline transcription promoters located at 5â² of the relevant constant (C)-distal J segments showed a strong conservation of the redundant STAT consensus motifs, indicating that TRG1@ and TRG2@ loci are under the influence of IL-7 and STAT signalling. These findings support the phylogenetic analysis of human and Bovidae (cattle and sheep) that revealed a different grouping pattern of C-distal compared to C-proximal J segments. Likewise, the phylogenetic behaviour of either C-distal and C-proximal J segments is in accordance with the Bovidae TRG clusters evolution. Comparison of sheep and human structures of recombination signal sequences (RSS) has highlighted a greater conservation in sheep 12 RSS rather than 23 RSS thus suggesting that the initial recruitment of recombination activating genes (RAG) products requires at least one relatively high-affinity RSS per recombination event.
Keywords
RT-PCRmaximum parsimony methodPB,IL-7RIL-7RAGIgHNMDPTCRSSbootstrap probabilityinterleukin-7recombination signal sequencesGenome evolutionneighbor-joining methodImmunoglobulin heavy chainnonsense-mediated mRNA decaySTAT proteinsJoiningRecombination activating genespremature termination codonInterleukin-7 receptor
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Genetics
Authors
M.C. Miccoli, G. Vaccarelli, C. Lanave, E.P. Cribiu, S. Ciccarese,