Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9127163 | Gene | 2005 | 8 Pages |
Abstract
While the current literature suggests that F116 is a non-integrating phage that maintains itself as a plasmid during the lysogenic life cycle, a putative int gene was identified. Of the phage structural genes, only the portal protein could be identified by homology. Analysis of F116 structural protein by one-dimensional SDS-PAGE revealed approximately 15 bands. MALDI-TOF MS analysis identified the gene encoding the major capsid protein. This protein appears to undergo posttranslational cleavage giving rise to a smaller capsid protein.
Keywords
RBSmultiple em for motif elicitationPCIPAGEPodoviridaeTPsattBCaudoviralesXgalRNaseORFIGR5-bromo-4-chloro-3-indolyl-β-d-galactosideSDSMEMEDNAAmpicillinBasic Local Alignment Search ToolEDTAEthylenediaminetetraacetic aciddeoxyribonucleic acidpolyacrylamide gel electrophoresisBlastTE bufferBacteriophageSingle strandedbase pairsDouble strandedribonucleaseribosome binding sitesodium dodecyl sulphateMass spectrometryPhageEvolutionopen reading frameLuria-Bertani (medium)MALDI-TOF MSIntergenic regionElectron microscopycapsid proteinpolyethylene glycolPEGkilobases
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Authors
Michaela Byrne, Andrew M. Kropinski,