Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9127351 | Gene | 2005 | 9 Pages |
Abstract
1A6/DRIM (Down-regulated in Metastasis) has been reported to express at a high level in the gastric cancer tissues and the premalignant lesions implicating the involvement of 1A6/DRIM in cell transformation. Although the information regarding the putative functions and distribution of the 1A6/DRIM in different tissues and cell lines has been increasing recently, its promoter and promoter-regulating factors remain unknown. In this study, the transcription initiation site of 1A6/DRIM was confirmed to be located at 147 bp upstream of the ATG codon using the primer extension analysis. The minimal promoter region of the 1A6/DRIM is located between â47 and +42 of the transcription initiation site measured by luciferase reporter assays using a set of deletion constructs. In addition, an E-box is shown to be an essential element for transcriptional regulation of 1A6/DRIM demonstrated by luciferase assay with different deletion and mutation constructs. Finally, a transcription factor, upstream stimulatory factor 2 (USF2) was found to be an activator of the 1A6/DRIM through binding to the E-box demonstrated by luciferase reporter assay, electrophoretic mobility shift assay, and chromatin immunoprecipitation (ChIP) assay. The structural analysis of the 1A6/DRIM promoter and the identification of its potential regulatory effecter may help us to understand its biological functions in regulating cancer development.
Keywords
HLHEMSAUSFSV40PMSFDTTFBSElectrophoretic mobility shift assayESTchromatin immunoprecipitationTranscriptional regulationTumorigenesisExpressed Sequence TagE-boxdithiothreitolfetal bovine serumUpstream stimulatory factorfluorescent in situ hybridizationphenylmethylsulfonyl fluorideFishpolymerase chain reactionPCRSimian virus 40CHiP
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Genetics
Authors
Xiaoyan Xing, Xiaojuan Du, Zheming Lu, Tao Ning, Xiulan Su, Yang Ke,