Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9138885 | Journal of Structural Biology | 2005 | 9 Pages |
Abstract
Energy-filtering transmission electron microscopy (EFTEM) allows the determination of elemental distributions out of a sequence of energy filtered images. Combined with electron tomography, EFTEM is a powerful tool to obtain three-dimensional chemical maps from sub-cellular structures. However, there is no existing software in the public-domain for the computation and analysis of 3D-chemical maps. Here, we present a Java-based program to compute 3D-elemental distribution. This program is available as a set of plug-ins for the public-domain Java image processing program ImageJ inspired by NIH Image. Its implemented algorithms have been successfully applied to the three-dimensional localization of iron granules in semi thin (200Â nm) epon sections from the vent worm Riftia pachyptalia.
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Authors
T. Boudier, J.P. Lechaire, G. Frébourg, C. Messaoudi, C. Mory, C. Colliex, F. Gaill, S. Marco,