Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9138944 | Journal of Structural Biology | 2005 | 5 Pages |
Abstract
The native polysomes of Saccharomyces cerevisiae were visualized in liquid solution by atomic force microscopy without external contrasting, such as shadowing and negative staining. This study showed native polysomes as lined particle with a height of ca. 27Â nm, which is agreement with the height of 80S ribosomes in previous study. We found a small subparticle, located in a ring-shape or at the end of a linear structure, and visualized mRNA chains between adjacent ribosomes. Although the structures of polysomes have been studied for decades, it has remained difficult to visualize the native three-dimensional form. By the observation in liquid solution, we temporarily stopped the translation using an antibiotic to presenting the native three-dimensional structure and function of the polysomes. Our results provide not only new findings on native eukaryotic polysomes, but also great potential to visualize the influence of various environmental conditions on polysomes.
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Authors
Eriko Mikamo, Chikako Tanaka, Takashi Kanno, Hideo Akiyama, Giman Jung, Hiroyuki Tanaka, Tomoji Kawai,