Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9139139 | Journal of Structural Biology | 2005 | 5 Pages |
Abstract
The RNA-dependent RNA polymerase, QDE-1, is a component of the RNA silencing pathway in Neurospora crassa. The enzymatically active carboxy-terminal fragment QDE-1 ÎN has been expressed in Saccharomyces cerevisiae in the presence and absence of selenomethionine (SeMet). The level of SeMet incorporation was estimated by mass spectrometry to be â¼98%. Both native and SeMet proteins were crystallized in space group P21 with unit cell parameters a = 101.2, b = 122.5, c = 114.4 Ã
, β = 108.9°, and 2 molecules per asymmetric unit. The native and SeMet crystals diffract to 2.3 and 3.2 Ã
, respectively, the latter are suitable for MAD structure determination.
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Authors
Minni R.L. Laurila, Paula S. Salgado, Eugene V. Makeyev, Joanne Nettelship, David I. Stuart, Jonathan M. Grimes, Dennis H. Bamford,