Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9139944 | Molecular and Biochemical Parasitology | 2005 | 10 Pages |
Abstract
By scanning all available Trypanosoma brucei cDNAs for splicing and polyadenylation sites, we found that trans splicing generally occurs at the first AG following a polypyrimidine tract of 8-25Â nt, giving rise to 5â²-UTRs of a median length of 68Â nt. We also found that in general, polyadenylation occurs at a position with one or more A residues located between 80 and 140Â nt from the downstream polypyrimidine tract. These data were used to calibrate free parameters in a grammar model with distance constraints, enabling prediction of polyadenylation and trans splice sites for most protein-coding genes in the trypanosome genome. The data from the genome analysis and the program are available from: http://web.cgb.ki.se/daniel/splicemodel.php.
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Authors
Corinna Benz, Daniel Nilsson, Björn Andersson, Christine Clayton, D. Lys Guilbride,