Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9142203 | Molecular Immunology | 2005 | 8 Pages |
Abstract
The M-CSF and its receptor (M-CSFR, CSF-1R or c-fms proto-oncogene) system were initially implicated as essential in mammals for normal monocyte development as well as for pregnancy. To allow a comparison with the M-CSF and M-CSFR system of an oviparous animal, we cloned a M-CSFR-like gene from rainbow trout (Oncorhynchus mykiss). The gene was cloned from a cDNA library of head kidney. It contained an open reading frame encoding 967 amino acids with a predicted size of 109Â kDa. The putative amino acid sequence of rainbow trout M-CSFR showed 54% amino acid identity to fugu (Takifugu rubripes) M-CSFR, 52% to zebrafish (Danio rerio) M-CSFR and 40% to mouse (Mus musculus) and human (Homo sapiens) M-CSFR. The M-CSFR-like gene was constitutively expressed in head kidney, kidney, intestine, spleen and blood. The gene was detected especially in the ovary of immature female rainbow trout. These results suggest that a M-CSFR-like receptor may be involved in female reproductive tracts even in an oviparous animal like fish.
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Authors
Teruko Honda, Takashi Nishizawa, Maya Uenobe, Chie Kohchi, Akashi Kuroda, Mitsuru Ototake, Teruyuki Nakanishi, Yuichi Yokomizo, Yukinori Takahashi, Hiroyuki Inagawa, Gen-Ichiro Soma,