Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9230608 | Journal of Investigative Dermatology | 2005 | 12 Pages |
Abstract
Langerin/CD207 is a C-type lectin associated with formation of Birbeck granules (BG) in Langerhans cells (LC). Here, we describe a monoclonal antibody (mAb 205C1) recognizing the extracellular domain of mouse langerin. Cell-surface langerin was detected in all epidermal LC, which presented a uniform phenotype. Two subpopulations of langerin+ cells were identified in peripheral lymph nodes (LN). One population (subset 1) was CD11clow/+/CD8αâ/low/CD11b+/CD40+/CD86+. The other population (subset 2) was CD11chigh/CD8α+/CD11blow, and lacked CD40 and CD86. Only subset 1 was fluorescein 5-isothiocyanate (FITC+) following painting onto epidermis, and the appearance of such FITC+ cells in draining LN was inhibited by pertussis toxin. Mesenteric LN, spleen, and thymus contained only a single population of langerin+ DC, corresponding to peripheral LN subset 2. Unexpectedly, BG were absent from spleen CD8α+ DC despite expression of langerin, and these organelles were not induced by mAb 205C1. Collectively, we demonstrate that two langerin+ DC populations (subsets 1 and 2) co-exist in mouse lymphoid tissue. Subset 1 unequivocally identifies epidermal LC-derived DC. The distribution of subset 2 indicates a non-LC origin of these langerin+ cells. These findings should facilitate our understanding of the role played by langerin in lymphoid organ DC subsets.
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Authors
Patrice Douillard, Patrizia Stoitzner, Christoph H. Tripp, Valérie Clair-Moninot, Smina Aït-Yahia, Alex D. McLellan, Andreas Eggert, Nikolaus Romani, Sem Saeland,